50 SMRT cells of Drosophila teissieri GT53w PacBio RS II reads - SRA

SRX9552123: 50 SMRT cells of Drosophila teissieri GT53w PacBio RS II reads
1 PACBIO_SMRT (PacBio RS II) run: 163,482 spots, 148.3M bases, 623.4Mb downloads

Design: High Molecular Weight DNA extraction with QIAgen Blood and Tissue kit (Genomic-tip 100/G), sheared with 24 gauge blunt-end needle (Chakraborty et al. 2016 NAR), verified length distribution by FIGE. Library preparations using BluePippin 15-50kb size selection, standard PacBio 20kb library preparation without Covaris G-tube step, followed by final BluePippin 15-50kb size selection of SMRTbell templates. Sequenced 50 SMRT cells totalling ~25.1 Gb. Dtei runs served as titration/testing for future preps for other species, so loading concentrations varied widely.

Submitted by: Princeton University

Study: Drosophila teissieri strain:GT53w Genome sequencing and assembly

PRJNA647698 • SRP273736 • All experiments • All runs

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Drosophila teissieri de novo genome assembly using 15-50kb size-selected PacBio long reads. Libraries were prepared from high molecular weight DNA extracted from pooled virgin females of GT53w, and size-selected pre- and post-library preparation.

Sample: Drosophila teissieri GT53w

SAMN15594708 • SRS7096003 • All experiments • All runs

Organism: Drosophila teissieri

Library:

Name: R028-B01.1

Instrument: PacBio RS II

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 163,482 spots, 148.3M bases, 623.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR13106592	163,482	148.3M	623.4Mb	2020-11-22

ID:: 12483538

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